This article throws light upon the top six applications of polymerase chain reaction the top six applications are 1 pcr in clinical diagnosis 2 pcr in dna sequencing 3 pcr in gene manipulation and expression studies 4 pcr in comparative studies of genomes 5 pcr in forensic medicine and 6 pcr in comparison with gene cloning. The advent of the polymerase chain reaction pcr radically transformed biological science from the time it was first discovered mullis 1990 for the first time it allowed for specific detection and production of large amounts of dna pcr based strategies have propelled huge scientific endeavors . The polymerase chain reaction pcr has dramatically transformed scientific research and diagnostic medicine over the years pcr has become an indispensable and integral part of clinical and . The polymerase chain reaction and its expanding numbers of modifications have become a mainstay in diagnostic and research medicine the technique allows amplification of nucleic acid sequences both for the purposes of disease and pathogen detection and also for the preparation of hybridization probes and sequencing templates. Polymerase chain reaction pcr is an efficient and cost effective molecular tool to copy or amplify small segments of dna or rna pcr combines the principles of complementary nucleic acid hybridization with those of nucleic acid replication that are applied repeatedly through numerous cycles
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